研究生学术报告预告登记(开题、中期、答辩)

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报告人: 张超
学号: 1017214033
学院: 环境科学与工程学院
报告类型: 第二次学术报告
日期: 20 June 2019
时间: 1:20 PM
地点: 43B317
导师: 孙井梅
题目: Optimisation for assay of fluorescein diacetate hydrolytic activity in urban inland river sediments
内容提要:

Determination method of fluorescein diacetate (FDA) has been widely used as a rapid and sensitive method for the quantification of microbial activity.  However, as the current determination method of FDA is based on soil samples, there might be errors in its direct application to urban river sediments.  Therefore, determination method of FDA in urban river sediments was optimized to improve experiential reproducibility in the present study.  The optimized method describes quantitative measurement of the fluorescein produced when 1.8 g of fresh sediment is incubated with 50 mmol/L phosphate buffer solution (pH: 7.2) and 0.2 mL of 3 g/L FDA at 35 °C for 30-75 min under a rotation of 200 rpm.  Subsequently, 8 mL acetone was added to terminate the reaction.  The covariation coefficient of the optimized method ranged from 0.7% to 3.3%.  In addition, the FDA hydrolytic rate was positively correlated with pollutants (e.g. organic matter, labile organic matter, total nitrogen and acidified volatile sulfides in sediments). Moreover, the establishment of multiple linear regression model provided valid evidence for predicting microbial activity in the sediments.

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登记人: 张超
登记时间: Sunday, 29 August 2021, 9:31 PM