研究生学术报告预告登记（开题、中期、答辩）

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       为加强研究生学术交流活动，推进学术创新，特开通“研究生学术报告预告区”。我校研究生和教师可以在预告区及时发布和了解有关研究生学术报告的信息，届时参加。也可就某学术报告展开专题讨论与交流。

报告人: 邱宣
学号: 2010213025
学院: 药物科学与技术学院
报告类型: 其他学术报告
日期: 2012年04月25日
时间:
地点: 24楼A区107
导师: 万谦宏
题目: Sensitive quantification of roflumilast and roflumilast N-oxide in human plasma
内容提要: A high throughput bioanalytical method based on semi-automated liquid extraction and liquid
chromatography–tandem mass spectrometry (LC–MS/MS) has been developed for the sensitive quantification of roflumilast and its metabolite roflumilast N-oxide, a phosphodiesterase (PDE) inhibitor in human plasma and serum. The sample work-up procedure comprised liquid extraction using penta-deuterated analogues of both analytes as internal standards. Chromatography was performed on C18 revered phase analytical columns at a flow rate of 0.5 mL/min in the dual column mode employing a column switching technique and a linear gradient from 18% to 54% acetonitrile in 0.005 M aqueous ammonium acetate containing 0.006% formic acid. Mass spectrometry was performed on an API 4000 instrument in the positive ion SRM-mode (selected reaction monitoring) with the Turbo-V® ionspray interface. The method showed linear detector responses over the entire calibration range between 0.1 ng/mL (lower limit of quantification
(LLOQ)) and 50 ng/mL (upper limit of quantification (ULOQ)) for both analytes. Linear regression
analysis with concentration-squared weighting (1/x2 for roflumilast and 1/x for roflumilast N-oxide)
yielded inaccuracy and precision values <15% and coefficients of correlation (r) for the calibration curves >0.99 for both analytes.
图片:
登记人: 邱宣
登记时间: 2012年04月24日 星期二 16:29
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