| 内容提要: |
Antibiotic resistance genes (ARGs) may lead to bacterial resistance and using antibiotics will promote ARGs spread. Large amount of antibiotics were used in aquaculture, but little attention were paid to the antibiotic resistant in fish gut. In this report, nine kinds of Chinese freshwater and marine fish were acquired in a city of northern China to test the amount of antibiotics and ARGs residues in their intestinal contents. The results showed that 4 kinds of antibiotics were detected from the intestinal contents, including Doxycycline (DOX), Tetracycline (TC), Sulfamethoxazole (SMX) and Roxithromycin (ROX), and the antibiotics with the largest detected amount was ROX in Sardinops sagax (2.83μg/kg). Ten kinds of ARGs were detected from the intestinal contents, including strA, strB, ermB, blaTEM, oxa-30, qnrB, qnrD, sul1, sul2 and tetB, as well as one type of integron intI1. The most abundant ARGs was blaTEM. Correlation analysis showed huge difference between freshwater fish and marine fish. The results can improve our understanding of the antibiotics and ARGs residues in edible fish. |