| 内容提要: |
This study comprehensively investigated the impacts of acetate/oleic acid ratio on sequencing batch reactor (SBR) with regard to the variations of reactor performance, quorum sensing (QS) and microbial community. Four duplicated SBR were used to conduct this experiment fed with different acetate/oleic acid ratios of 80/20% (R1), 60/40% (R2), 40/60% (R3), and 20/80% (R4) in terms of chemical oxygen demand (COD) concentration. The results showed that the removal of NH+ 4-N was not affected by the acetate/oleic acid ratio, while the removal of COD, total nitrogen (TN) and PO3- 4-P were significantly affected, overall decreasing with the oleic acid ratio increasing. Growing ratios of oleic acid led to more serious sludge bulking, which was significantly positively correlated with protein/polysaccharide (p < 0.001). The correlations of QS with SBR performance and sludge properties were also observed. High-throughput sequencing demonstrated that the microbial compositions shifted significantly with varying acetate/oleic acid ratios, which especially contributed to significant differences in the abundance of Candidatus Competibacter, Denitratisoma and Zoogloea. Moreover, the potential correlations of bacterial genera with SBR performance and QS were also proposed, suggesting Candidatus Competibacter, f_Caldilineaceae_Unclassified, Falsirhodobacter, f_PHOS_HE36_Unclassified and Pseudorhodobacter were likely the dominant AHLs producers. The present work elucidated the effects of acetate/oleic acid ratio on SBR from a microbial viewpoint, which provided new insight into fully understanding of the essential roles of carbon source on wastewater treatment. |