| 内容提要: |
Blood-contacting materials with anti-platelet aggregation and anticoagulant properties are in great demand in biomedical field. Herein, hydrophilic poly(ethylene glycol) (PEG) and antithrombotic peptide ACH11 were co-immobilized onto Au to develop a multifunctional surface with remarkable hemocompatibility, anti-protein adsorption, anti-platelet aggregation, anticoagulant properties and good histocompatibility. PEG can not only help the surface resist non-specific protein adsorption and improve its hemocompatibility, but also benefit for the immobilized ACH11 to maintain its bioactivity in plasma. The anticoagulant peptide ACH11 can endow the surface with the ability of activated coagulation factor Xa (FXa) inhibition and anti-platelet aggregation activities. Au was first aminated with 2-aminoethanethiol (AET) through strong Au-S bond, and then reacted with PEG-ACH11 through active ester chemistry to prepare the multifunctional surface Au-PEG-ACH11, among which the heterobifunctional PEG (OPSS-PEG-NHS) served as a linker to immobilize antithrombotic ACH11. |