As a core of chromatographic separation system, the chromatographic column packings performance is related to the column efficiency, selectivity and separation degree. So it is the basis of the research and development to prepare, select and applicate a suitable chromatographic packings, including different separation modes and preparation methods.
Therefore, the study of liquid chromatographic packing was the most dynamic and the most creative work.
Reversed-phase liquid chromatography (RPLC) is one of the most popular and powerful analytical separation methods.
A recent survey has shown that approximately 60% of all commercial high-performance liquid chromatography columns sold are reversed-phase columns .
So, we will prepare the RPLC based on the silica matrix. |