研究生学术报告预告登记(开题、中期、答辩)

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报告人: 张博禹
学号: 2020214115
学院: 环境科学与工程学院
报告类型: 第一次学术报告
日期: 2020年09月27日
时间: 13:30
地点: 线上报告
导师: 石家福
题目: Oriented immobilization of enzyme–DNA conjugates on magnetic Janus particles for constructing a multicompartment multienzyme system with high activity and stability
内容提要:

Various organelles (e.g., mitochondria and chloroplasts) have a multicompartment structure, providing superior function of material transformation, selective segregation and energy conversion. Enlightened by the elegant evolution of nature, intended isolation of the biochemical process by cooperative multicompartments in cells has become an appealing blueprint to construct bioreactors. In this study, we develop a "soft separation" way to establish a delicate multicompartment multienzyme system (MMS) with polyphenol-encapsulated enzyme-DNA conjugates, which are anchored on magnetic Janus particles, providing a biomimetic catalysis network with the model cascade reactions in confinement. The well-designed MMS exhibits preferable bioactivity benefitting from the dependable DNA bridges and the oriented immobilization of enzymes, while the polyphenol shell further protects the anchored enzymes from exterior attacks, such as heat and enzymatic degradation. Moreover, by applying the MMS as nanomotors, the asymmetrical distribution of enzymes on Janus particles is found to improve mutual elevation between the self-driven locomotion and enzyme-mediated reactions, delivering enhanced dispersal ability and bioactivity. Owing to the excellent enzymatic activity, promoted stability and satisfying biocompatibility, the assembled MMS is proved to be promising for thein vitroand intracellular sensing of glucose, showing significant potential for biochemical analysis applications.

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登记人: 张博禹
登记时间: 2021年03月12日 星期五 12:39